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Santa Cruz Biotechnology
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Boehringer Mannheim
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Image Search Results
Journal: Developmental dynamics : an official publication of the American Association of Anatomists
Article Title: Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract.
doi: 10.1002/dvdy.75
Figure Lengend Snippet: Fig. 11. Cdc42 fails to localize properly in the CNCC of Ednra-/- embryos. Sagittal sections
Article Snippet: Culture medium was changed before making the scratch and 100nM Edn1 (Calbiochem) added with 3μM BMS 182874 Ednra inhibitor (
Techniques:
Journal: Developmental dynamics : an official publication of the American Association of Anatomists
Article Title: Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract.
doi: 10.1002/dvdy.75
Figure Lengend Snippet: Fig. 13. Analysis of CNCC migration in the E9.0 cultured embryos treated with the Cdc42
Article Snippet: Culture medium was changed before making the scratch and 100nM Edn1 (Calbiochem) added with 3μM BMS 182874 Ednra inhibitor (
Techniques: Migration, Cell Culture
Journal: Nature communications
Article Title: Polarized NHE1 and SWELL1 regulate migration direction, efficiency and metastasis.
doi: 10.1038/s41467-022-33683-1
Figure Lengend Snippet: Fig. 5 | Cdc42 facilitates the reversal of migration direction by controlling NHE1 repolarization. a Migration velocity and b fraction of vehicle control and ML141- treated MDA-MB-231 cells displaying negative velocity before (gray) and after (red) hypotonic shock (165 mOsm/l) at the leading edge in confinement. Data represent the a mean ± SD for the indicated number of cells or b mean ± SEM from 3 (a) or 4 (b) independent experiments. c Representative images and d quantification of NHE1 polarization in vehicle control and ML141-treated MDA-MB-231 cells inside confining channels before (340 mOsm/l) and after (165 mOsm/l) application of hypotonic shock at the leading edge. NHE1 is polarized at the cell leading edge in control and ML141-treated cells before osmotic shock (yellow arrowheads) and repolarizes to the “new” leading edge in response to osmotic shock only in control (red arrowhead), but not ML141-treated (white arrowhead),cells. Data represent the mean ± SD from three independent experiments. e Time-lapse montage of an MDA-
Article Snippet: In select experiments, cells were treated with the following pharmacological agents or corresponding vehicle controls: 5-(N-Ethyl-N-isopropyl) amiloride (EIPA, 40 μM, Sigma–Aldrich), DCPIB (40 μM, Tocris Biosciences), bumetanide (30μM, Santa Cruz Biotechnology), Lat-A (2 μM, Sigma–Aldrich),
Techniques: Migration, Control